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  • The replication clamp-loading machine at work in the three

    Sep 06, 2006 · The single clamp loader within the Pol III holoenzyme studies of subunit arrangement and functional consequences. A conserved domain of the large subunit of replication factor C binds PCNA

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  • Subunit Interaction Differences Between the Replication

    Replication Factor C Clamp Loader Subunit Arrangement within the Circular Pentamer and Its Attachment Points to Proliferating Cell Nuclear Antigen.pdf Content …

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  • Replication Factor C Clamp Loader Subunit Arrangement

    Replication Factor C - an overview | ScienceDirect Topics

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  • RFC3 - Replication factor C subunit 3 - Saccharomyces

    Replication factor C (RFC) is a heteropentamer of subunits RFC1, RFC2, RFC3, RFC4 and RFC5 and forms a complex with POL30/PCNA in the presence of ATP. Component of the RAD24-RFC complex which consists of RAD14, RFC2, RFC3, RFC4 and RFC5 and associates with the checkpoint clamp DDC1:MEC3:RAD17 complex.

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  • articles Structural analysis of a eukaryotic sliding DNA

    crystal structure of the five-protein clamp loader complex (replication factor-C, RFC) of the yeast Saccharomyces cerevisiae, RFC results in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring. Placement of a model for Each clamp loader subunit contains two structurally conserved

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  • 1sxj - Proteopedia, life in 3D

    Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA) PDB ID …

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  • Clamp Loader ATPases and the Evolution of DNA Replication

    (c) Bacteriophage clamp loaders consist of two distinct proteins. The gp62 protein, which lacks a AAA+ module but has an A' domain similar to that of eukaryotic clamp loaders, lies at the A position. The ATPase gp44 protein lies at the B, C, D, and E positions. Archaeal clamp loaders have a similar composition (see text for details).

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  • Mapping the interaction of DNA with the Escherichia coli

    184 VOLUME 12 NUMBER 2 FEBRUARY 2005 NATURE STRUCTURAL & MOLECULAR BIOLOGY ized (reviewed in refs. 7,21). The E. coli clamp loader minimally consists of one δ A subunit, three identical γ subunits (γ B, γ C and γ D, collectively referred to as γ BCD) and one δ′ E subunit. The δ A subunit binds to the β clamp and opens one of the clamp's two intermo-

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  • (PDF) Replication Factor C Clamp Loader Subunit

    Replication Factor C - an overview | ScienceDirect Topics

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  • Replication Clamps and Clamp Loaders

    The eukaryotic clamp loader, referred to as replication factor C (RFC) or activator 1, is highly conserved from yeast to humans and is composed of five distinct subunits, RFCs 1–5, all of which share a similar three-domain core architecture reminiscent of their ancestral counterparts (Burbelo et al. 1993; Cullmann et al. 1995; Uhlmann et al

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  • Figure 14.1 from The RFC clamp loader: structure and

    Structural studies reveal clamp loaders to be heteropentamers. Each subunit contains a region of homology to AAA+ proteins that defines two domains. The AAA+ domains form a right-handed spiral upon binding ATP. This spiral arrangement generates a DNA binding site within the center of RFC. Expand. View on PubMed.

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  • Replication Factor C - an overview | ScienceDirect Topics

    Dec 12, 2003 · Replication factor C (RFC) is a heteropentameric AAA+ protein clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor. The prokaryotic homologue, γ complex, is also a heteropentamer, and structural studies show the subunits are arranged in a circle.

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  • Improved solubility of replication factor C (RFC) Walker A

    Jun 01, 2012 · Replication factor C clamp loader subunit arrangement within the circular pentamer and its attachment points to proliferating cell nuclear antigen J. Biol. Chem., 278 ( 2003 ), pp. 50744 - 50753 Article Download PDF View Record in Scopus Google Scholar

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  • A Central Swivel Point in the RFC Clamp Loader Controls

    The eukaryotic clamp loader replication factor C (RFC) loads proliferating cell nuclear antigen (PCNA) clamps onto DNA, where they serve as processivity factors for replicative polymerases and modulate the functions of many other proteins that work on DNA.1,2 The structure and function of clamp and clamp loader proteins are highly con-

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  • Clamp loaders and sliding clamps David Jeruzalmi, Mike O

    The bacteriophage T4 clamp loader is also constituted of five subunits (four copies of the gp44 ATPase and one copy of gp62) [42,43]. In eukaryotes, the clamp loader is known as RFC (replication factor C) and is composed of five distinct proteins (RFCs 1–5) [44]. In archaebacteria, the clamp loader is formed from two kinds of subunits, large

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  • Molecular Analyses of a Three-Subunit Euryarchaeal Clamp

    Aug 28, 2009 · Whereas the bacterial clamp loader is termed the γ-complex, the eukaryotic functional homolog is known as replication factor C or RFC (3, 19). Cullmann and coworkers demonstrated that the heteropentameric RFC complex in eukaryotes is encoded by five different genes: four different genes coding for the RFC small subunit (RFCS) proteins and the

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  • Replication factor C clamp loader subunit arrangement

    Replication Factor C - an overview | ScienceDirect Topics

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  • OMIM Entry - * 102579 - REPLICATION FACTOR C, SUBUNIT 1; …

    Crystal Structure. Bowman et al. (2004) reported the crystal structure of the 5-protein clamp loader complex (replication factor-C, RFC) of the yeast S. cerevisiae, bound to the sliding clamp (proliferating cell nuclear antigen, or PCNA). Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA

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  • The replication factor C clamp loader requires arginine

    Sep 15, 2006 · Replication factor C clamp loader subunit arrangement within the circular pentamer and its attachment points to proliferating cell nuclear antigen. Yao N, Coryell L, Zhang D, Georgescu RE, Finkelstein J, Coman MM, Hingorani MM, O'Donnell M. J Biol Chem, 278(50):50744-50753, 06 Oct 2003

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  • (PDF) Communication between subunits within an archaeal

    Communication between subunits within an archaeal clamp-loader complex. We have investigated the communication between subunits in replication factor C (RFC) from Archaeoglobus fulgidus

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